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Publications

Publications

2007

• Wong, T. S., Roccatano, D., Schwaneberg, U. (2007). Steering directed protein evolution: Strategies to manage combinatorial complexity of mutant libraries. Environmental Microbiology, in press.
• Kuper. J., Wong, T. S., Roccatano, D., Wilmanns, M., Schwaneberg, U. (2007) Understanding the mechanism of organic co-solvent inactivation in heme monooxygenase P450 BM-3, JACS, 129, 5786-5787.
• Zhu, Z., Momeu, C., Nazor, G., Prodanovic, R., Schwaneberg, U. (2007). Directed evolution of glucose oxidase for mediated electron transfer, Biotechnol. J., 2, 241-248.
• Tee, K. L. and Schwaneberg, U. (2007). Directed evolution of oxygenases: screening systems, success stories and challenges, Comb. Chem. High Throughput Screen, 10, 197-217 (review).
• Wong, T. S., Roccatano, D., Schwaneberg, U. Genetic code’s challenges for exploring sequence space in directed protein evolution, Biocatal. Biotrans., in press.
• Wong, T. S., Roccatano, D., Schwaneberg, U. (2007). Are transversion mutations better? A Mutagenesis Assistant Program analysis on P450 BM-3 heme domain, Biotechnol. J., 2, 133-142.

2006

• Tee, K. L. and Schwaneberg, U. (2006). Gelenkte Evolution of Epoxygenasen Chemie Ingenieur Technik, 78, 1438-1439.
• Roccatano, D., Wong, T. S., Schwaneberg, U., Zacharias, M. (2006) Toward understanding the inactivation mechanism of monooxygenase P450 BM-3 by organic cosolvents: a molecular dynamics simulation study, Biopolymers, 83, 467-476 (cover page).
• Onaca, O., Nallani, M., Ihle, S., Schenk, A. and Schwaneberg, U. (2006) Functionalized nanocompartments (Synthosomes): limitations and prospective applications in industrial biotechnology, Biotechnology Journal, 7-8, 795-805.
• Tee, K. L. and Schwaneberg, U. (2006) A screening system for directed evolution of epoxygenases: validation reveals importance of position 184 in P450 BM3 for stereoselective styrene epoxydation, Angewandte Chemie, 45(32):5380-5383.
• Nallani, M., Onaca, O., Hoheisel, W., and Schwaneberg, U. (2006). A nanophosphors based method for selective DNA recovery in Synthosomes, Biotechnology Journal, 7-8, 828-834.
• Schenk, A., Wong, T. S., Roccatano, D., Hauer, B. and Schwaneberg, U. (2006). SeSaM (Sequence Saturation Mutagenesis): Eine Methode zur Saettigungsmutagenese eines Genes, BIOspektrum, 3 , 277-279.
• Nazor, J., and Schwaneberg, U. (2006). Laboratory evolution of P450 BM-3 for mediated electron transfer ChemBioChem, 7, 638-644.
• Nallani, M., Graf, A., Onaca, O., Lindemann, M., Winterhalter, M, Meier, W. and Schwaneberg, U. (2006) A nanocompartment (nanocontainer) system suitable for biotechnological applications, J. Biotechnol., 123, 50-59.
• Wong, T. S., Roccatano, D., Zacharias, M. and Schwaneberg, U. (2006). A statistical analysis of current random mutagenesis methods for directed protein evolution, J. Mol. Biol. 355, 858-871 (cover page).
• Zhu, Z., Momeu, C., Zakhartsev, M., Hernandez, J. C. and Schwaneberg, U. (2006). Making glucose oxidase fit for biofuel cell applications by directed protein evolution, Biosens. & Bioelectron, 21, 2046-2051.
• Wong, T. S., Zhurina, D. and Schwaneberg, U. (2006). The diversity challenge in directed protein evolution, Comb. Chem. High Throughput Screen., 9, 271-289.
• Wong, T. S., Schwaneberg, U., Hauer, B. and Breuer, B. (2006). A filter-paper based assay for laboratory evolution of hydrolases and dehydrogenases, Comb. Chem. High Throughput Screen., 9, 289-293.

2005

• Roccatano, D., Wong, T. S., Schwaneberg, U., Zacharias, M. (2005). Structural and dynamic properties of cytochrome P450 BM-3 in pure water and in dimethylsulphoxide/water mixture, Biopolymers 78, 259-267.
• Wong, T. S., Wu, N., Roccatano, D., Zacharias, M. and Schwaneberg, U. (2005). Sensitive assay for laboratory evolution of hydroxylases toward aromatic and heterocyclic compounds, J. Biomol. Screen. 10, 246-252 (cover page).
• Wong, T. S., Tee, K. L., Hauer, B. and Schwaneberg, U. (2005). Sequence Saturation Mutagenesis with tunable mutation frequencies, Anal. Biochem. 341, 187-189.

2004

• Wong, T. S., Tee, K. L. and Schwaneberg, U. (2004). Sequence Saturation Mutagenesis (SeSaM): A novel method for directed evolution, Nucleic Acids Res. 32, e26.
• Wong, T. S., Arnold, F. H. and Schwaneberg, U. (2004). Laboratory evolution of cytochrome P450 BM-3 monooxygenase for organic co-solvents, Biotechnol. and Bioeng. 85, 351-358.

2003

• Wong, T. S., and Schwaneberg, U. (2003). Protein Engineering in Bioelectrocatalysis, Curr. Opin. Biotechnol. 14, 590-596.

Before Jacobs University Bremen (1995 - 2001)

• Farinas, E., Schwaneberg, U., Anton, G. and Arnold, F. H. (2001). Directed evolution of a cytochrome P450 monooxygenase for alkane oxidation. Adv. Synth. Catal. 343, 601-606.
• Appel, D., Lutz-Wahl, S., Fischer, P., Schwaneberg, U. and Schmid, R. D. (2001). Hydroxylation of alkanes, cycloalkanes, arenes and heteroarenes by a P450 BM-3 mutant, evolved by directed evolution, J. Biotechnol. 88, 167-171.
• Lentz, O., Li, Q.-S., Schwaneberg, U., Lutz-Wahl, S., Fischer, P. and Schmid, R. D. (2001). Modification of the fatty acid specificity of cytochrome P450 BM-3 from Bacillus megaterium by directed evolution: a validated assay, J. Mol. Catal. B-Enzym 629, 1-11.
• Schwaneberg, U., Cirino, P. C., Otey, C., Farinas, E. and Arnold, F. H. (2001). Cost-effective whole cell assay for laboratory evolution of hydroxylases in E. coli, J. Biomol. Screen.6, 111-118.
• Li, Q.-S., Schwaneberg, U., Fischer, M., Schmitt, J., Pleiss, J. and Schmid, R. D. (2000). Rational evolution of a medium chain-specific cytochrome P450 BM-3 variant, Biochem. Biophys. Acta, 1545, 114-121.
• Schwaneberg, U., Appel, D., Schmitt, J. and Schmid, R. D. (2000). P450 in biotechnology. Zinc driven -hydroxylation of p-nitrophenoxydodecanoic acid using P450 BM-3 F87A, J. Biotechnol. 84, 249-257.
• review article in the book: Enzymes in lipid modification. Wiley-VCH-Verlag, Weinheim. Schwaneberg, U. and Bornscheuer, U. (2000). Fatty acid hydroxylations using P450 monooxygenase, 396-416.
• Li, Q.-S., Schwaneberg, U., Fischer, P. and Schmid, R. D. (2000). Directed evolution of the fatty-acid hydroxylase P450 BM-3 into an indole-hydroxylating catalyst, Chem. Eur. J. 6, 1531-1536.
• Schwaneberg, U., Sprauer, A., Schmidt-Dannert, C. and Schmid, R. D. (1999). P450 monooxygenase in biotechnology. 1. Single step, large scale purification method for cytochrome P-450 BM-3 by anion exchange chromatography, J. Chromatogr. A 848, 149-159.
• Schwaneberg, U., Schmidt-Dannert, C., Schmitt, J. and Schmid, R. D. (1999). A continuous spectrophotometric assay for P-450 BM-3, a fatty acid hydroxylating enzyme, and its mutant F87A, Anal. Biochem. 269, 359-366.
• Trummler, K., Roos, J., Schwaneberg, U., Effenberger, F., Foerster, S., Pfizenmaier, K. and Wajant, H. (1998). Expression of the Zn2+-containing hydroxynitrile lyase from Flax (Linum usitatissimum) in Picha pastoris, Plant Science 139, 19-27.
• Wuensche, K., Schwaneberg, U., Bornscheuer, U. and Meyer, H. H. (1996). Chemo-enzymic route to b-blockers via 3-hydroxy esters, Tetrahedron Asymmetry 7, 2017-2022.
• Lau, R., Schuele, G., Schwaneberg, U. and Ziegler, T. (1995). Intramolecular glycosylation of prearranged saccharides as a novel strategy for the construction of b-L-rhamnosidic linkages, Liebigs Ann. 10, 1745-1754.

Patents

• Nallani, M., Meier, W., Winterhalter, M. and Schwaneberg, U.
  Geladene Vesikel
  Filed in 02/2004 (EP)
• Wong, T. S., Hauer, B. and Schwaneberg, U.
  A process for Sequence Saturation Mutagenesis (SeSaM)
  Filed in 10/2003 (WO)
• Schwaneberg, U. and Arnold F.
  Laboratory evolution of monooxygenase P450 BM-3 for industrial applications
  CIT-3515
• Farinas, E., Glieder, A., Arnold, F. and Schwaneberg U.
  Directed evolution of a cytochrome P450 monooxygenase for alkane oxidation
  CIT-3514
• Arnold, F., Joern, J., Sakamoto, T. and Schwaneberg, U.
  Screens for the discovery and directed evolution of oxygenase enzymes
  CIT 00-83
• Lenz, O., Schwaneberg,U., Pleiss, J. and Schmid, R. D.
  Hydroxylierung von Carbonsaeuren mittlerer Kettenlaenge. (Hydroxylation of carboxylic acids with a medium chain length).
  BASF AG Ludwigshafen O. Z. 0050/051241 (2000)
• Li, Q.-S., Schwaneberg, U., Fischer, M., Pleiss, J. and Schmid, R. D. Neue Cytochrom P450-Monooxygenasen und deren Verwendung zur Oxidation N-heterocyclischer Aromaten. (Novel cytochrome P450 monooxygenases and their use in oxidation of N-heterocycles).
  BASF AG Ludwigshafen O. Z. 0050/050915 (1999)
• Schwaneberg, U., Appel, D., Hauer, B. and Schmid, R. D.
  Elektronendonorsystem fuer Enzyme. (Electron donor systems for enzymes).
  BASF AG Ludwigshafen, DE 199 35 115.5 (1999)